| Gene | Thtpa |
| Chromosome | 14 |
| Deletion Start | 55,095,444 |
| Deletion End | 55,097,541 |
| Deletion Size | 2,098 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,095,839 | 55,095,859 | CCTGGAAGCTGGCCTTATCTG |
| TUP | 55,095,861 | 55,095,884 | AGCCCATGGACAGGAGCCTCAGCT |
| TUR | 55,095,907 | 55,095,926 | CCCACAGCATAGCCGAAGTC |
| TDF | 55,097,304 | 55,097,323 | GGGTAGGCAGGGAACAGAAC |
| TDP | 55,097,324 | 55,097,345 | CGAAGCATGTCCGAGGGCTCCA |
| TDR | 55,097,360 | 55,097,377 | GCGGGCAAGAGGACAATG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCATTACGGCCTGTTTG |
| SD | ACGAGCGGAAATCAATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 288 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11573B-A9 | VGB6 | C57BL/6NTac | |
| 11573B-F12 | VGB6 | C57BL/6NTac | |
| 11573B-C3 | VGB6 | C57BL/6NTac | |
