| Gene | Phlda2 |
| Chromosome | 7 |
| Deletion Start | 143,502,487 |
| Deletion End | 143,501,718 |
| Deletion Size | 770 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,502,236 | 143,502,255 | ACTTCACCATCGTCACCAAC |
| TUP | 143,502,208 | 143,502,232 | TACAAGGAGATCGACTTCCGCTGCA |
| TUR | 143,502,187 | 143,502,205 | CGTTCCAGCAGCTCTCCAC |
| TDF | 143,501,885 | 143,501,902 | TGGAACGCATGGCAGGAG |
| TDP | 143,501,862 | 143,501,883 | TGCTCGGCCCACCTGGAGACTT |
| TDR | 143,501,820 | 143,501,842 | TAGGGAGAAGACTGAGTCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | GCGCGGTAGTTCAGTCTAGG |
| SD | CAAAGGCACTTTGAATGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11574A-H5 | VGB6 | C57BL/6NTac | |
| 11574A-F3 | VGB6 | C57BL/6NTac | |
| 11574A-B3 | VGB6 | C57BL/6NTac | |
| 11574A-D11 | VGB6 | C57BL/6NTac | |
