| Gene | Tnfrsf17 |
| Chromosome | 16 |
| Deletion Start | 11,313,937 |
| Deletion End | 11,319,958 |
| Deletion Size | 6,022 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,314,784 | 11,314,803 | ACCTGGACCACATTGTGAAC |
| TUP | 11,314,804 | 11,314,828 | TTCAGGGCAGCTTGGGCTAGAGTAA |
| TUR | 11,314,856 | 11,314,877 | ACCAACTCAGCTACAGCTCATC |
| TDF | 11,319,544 | 11,319,563 | GCGACTCTCAGTGCAGGATG |
| TDP | 11,319,564 | 11,319,588 | CACTCATCCTTGGTCTTGTGCTGCC |
| TDR | 11,319,593 | 11,319,611 | CAGCAGGACACAGCAGGAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGTCCACATTGCAACTGT |
| SD | TTTGGTTTTGGTTTAGAGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 416 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 424 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11580A-B4 | VGB6 | C57BL/6NTac | |
| 11580A-D4 | VGB6 | C57BL/6NTac | |
| 11580A-H5 | VGB6 | C57BL/6NTac | |
