| Gene | Dnaja3 |
| Chromosome | 16 |
| Deletion Start | 4,684,119 |
| Deletion End | 4,705,953 |
| Deletion Size | 21,835 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,684,306 | 4,684,324 | GGGCGCTGCTGACATTGAG |
| TUP | 4,684,327 | 4,684,346 | CCGGTGTCCGCCTCACAGGT |
| TUR | 4,684,388 | 4,684,405 | GAGCCGAATCGCCCTTTC |
| TDF | 4,705,807 | 4,705,827 | GCTCTTGACCTCGGTTCTTTC |
| TDP | 4,705,830 | 4,705,852 | TTGTTTGTGCCCAGGTGGCAGAA |
| TDR | 4,705,868 | 4,705,888 | CTCGCCTATCCTTGCTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | ACAAGTAAAGTGCAGCATTCG |
| SD | TGAGGACTGTAAGGTACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11581A-A10 | VGB6 | C57BL/6NTac | |
| 11581A-B10 | VGB6 | C57BL/6NTac | |
