| Gene | Unc119b |
| Chromosome | 5 |
| Deletion Start | 115,134,907 |
| Deletion End | 115,125,350 |
| Deletion Size | 9,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,133,999 | 115,134,021 | GTGTCTGCTCCCTGATGATCTAC |
| TUP | 115,133,969 | 115,133,990 | TTTGGAAGCTGCCCAGTCCAGC |
| TUR | 115,133,945 | 115,133,965 | GTGAGGAAAGGGTGTCAGGTC |
| TDF | 115,125,678 | 115,125,702 | GATGAGGCTAGGGATGACTAAAGAG |
| TDP | 115,125,650 | 115,125,676 | TGATTGCATCCTGGTGTAGAACTGGAC |
| TDR | 115,125,626 | 115,125,644 | GCGCCAGATGGACTGTGAC |
PCR Primers
| Primer | Sequence |
| SU | TATCCAGAGTGACTGGAGTG |
| SD | ACTTCACTATAATACCTCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 317 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 282 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11585B-A11 | VGB6 | C57BL/6NTac | |
| 11585B-D8 | VGB6 | C57BL/6NTac | |
| 11585B-F4 | VGB6 | C57BL/6NTac | |
| 11585B-G5 | VGB6 | C57BL/6NTac | |
| 11585C-B11 | VGB6 | C57BL/6NTac | |
