| Gene | Tmem35 |
| Chromosome | X |
| Deletion Start | 134,295,495 |
| Deletion End | 134,304,854 |
| Deletion Size | 9,360 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,295,737 | 134,295,756 | GGACCCTCCTCCCTAACAAC |
| TUP | 134,295,764 | 134,295,786 | CTCGGAAGGAACAGCTGGACCGA |
| TUR | 134,295,796 | 134,295,815 | TCCCAGCATAGCCCACAAAG |
| TDF | 134,304,551 | 134,304,570 | AAGTGGCCTGTGGCATTGTC |
| TDP | 134,304,572 | 134,304,593 | TGACCCTGGTACCCGGTCGTCC |
| TDR | 134,304,609 | 134,304,631 | ACACCAGCAAGAGCAGGAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TTAACTTTGGCAAGGCACCG |
| SD | CTTGCTGGAGTCCATCTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11586A-A5 | VGB6 | C57BL/6NTac | |
| 11586B-B9 | VGB6 | C57BL/6NTac | |
| 11586B-D4 | VGB6 | C57BL/6NTac | |
