| Gene | Gcm2 |
| Chromosome | 13 |
| Deletion Start | 41,109,951 |
| Deletion End | 41,102,732 |
| Deletion Size | 7,220 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,109,931 | 41,109,949 | AGCAGACAGCACGCAGGAC |
| TUP | 41,109,906 | 41,109,929 | AGGATGCTGTGCTCTCCTACGGGA |
| TUR | 41,109,867 | 41,109,886 | GAGGCATCTGTGGGTCATTG |
| TDF | 41,103,229 | 41,103,250 | GCCTTGAGGAGAAGGTTGACTG |
| TDP | 41,103,208 | 41,103,228 | TGAGCAATGCCAGGCTGTGCC |
| TDR | 41,103,174 | 41,103,192 | GCAGGGCAGCTCTAGGTTG |
PCR Primers
| Primer | Sequence |
| SU | CCTGCTTAAAGGTGCTTCTG |
| SD | GATATTGCAGGCTCCTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 658 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11589A-A3 | VGB6 | C57BL/6NTac | |
| 11589A-B6 | VGB6 | C57BL/6NTac | |
| 11589A-D10 | VGB6 | C57BL/6NTac | |
| 11589A-E3 | VGB6 | C57BL/6NTac | |
| 11589A-F9 | VGB6 | C57BL/6NTac | |
