| Gene | Bcl2l12 |
| Chromosome | 7 |
| Deletion Start | 44,996,981 |
| Deletion End | 44,991,366 |
| Deletion Size | 5,616 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,996,417 | 44,996,441 | CAGCACAAGCCATAACCATTTCAAG |
| TUP | 44,996,389 | 44,996,416 | TCTTTGTCACACCTCTTTCTAGCGGGTA |
| TUR | 44,996,360 | 44,996,381 | CCTCCTATCCAAGCACAAACAC |
| TDF | 44,991,653 | 44,991,671 | GGCAAGACAGGAGGGACAG |
| TDP | 44,991,629 | 44,991,651 | TAGAGCTCAGCGGCCCTGCTTCT |
| TDR | 44,991,562 | 44,991,585 | GACGGAGGTTAAGCAACACTGATG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAAGAGTTCAAGCACTC |
| SD | TGGGTTGTGATAAAGAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11591B-F11 | VGB6 | C57BL/6NTac | |
| 11591C-A3 | VGB6 | C57BL/6NTac | |
| 11591C-C2 | VGB6 | C57BL/6NTac | |
| 11591C-F3 | VGB6 | C57BL/6NTac | |
| 11591C-G9 | VGB6 | C57BL/6NTac | |
| 11591C-H5 | VGB6 | C57BL/6NTac | |
