| Gene | Sumo3 |
| Chromosome | 10 |
| Deletion Start | 77,606,324 |
| Deletion End | 77,616,260 |
| Deletion Size | 9,937 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,607,047 | 77,607,065 | TTGCCTGCGAGGTCCTTAC |
| TUP | 77,607,072 | 77,607,095 | TTTCAGCTCAGCCTTGGGTCCTGC |
| TUR | 77,607,115 | 77,607,135 | AGGGCGAACAGGTGCTCATTC |
| TDF | 77,616,095 | 77,616,119 | CACTTCTAACATTTCCTGTCTCCTC |
| TDP | 77,616,120 | 77,616,141 | TCCGGGCCCTCCTTGTCAACTT |
| TDR | 77,616,151 | 77,616,171 | GGTGTCCTCATCCTCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | TAGGACCTTGAGTCGCGCG |
| SD | GGTGAGAATCTACATCCAGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 437 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 365 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11592A-D10 | VGB6 | C57BL/6NTac | |
| 11592A-H1 | VGB6 | C57BL/6NTac | |
| 11592A-F5 | VGB6 | C57BL/6NTac | |
| 11592A-F7 | VGB6 | C57BL/6NTac | |
| 11592A-G4 | VGB6 | C57BL/6NTac | |
