| Gene | Gata3 |
| Chromosome | 2 |
| Deletion Start | 9,877,586 |
| Deletion End | 9,858,333 |
| Deletion Size | 19,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,876,716 | 9,876,736 | CACGGTCCAGGGAGAGGAAAG |
| TUP | 9,876,688 | 9,876,715 | CCTTAAGCTGAAGAGAAGTCGGGTTTGC |
| TUR | 9,876,667 | 9,876,684 | TCTGTGTAGCCGGGAGTC |
| TDF | 9,859,170 | 9,859,189 | AGAAGAGTGACAGCCAGACC |
| TDP | 9,859,146 | 9,859,168 | TGCAGGAAGGCACAGAGACCCAG |
| TDR | 9,859,109 | 9,859,131 | TGAAGCCTCCTGTGTTGAAATCC |
PCR Primers
| Primer | Sequence |
| SU | TTCTATACCCTTAACTGCAAAC |
| SD | GAGTCTGAATGGCTTATTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 263 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11593A-C2 | VGB6 | C57BL/6NTac | |
| 11593A-C7 | VGB6 | C57BL/6NTac | |
