| Gene | Hdgf |
| Chromosome | 3 |
| Deletion Start | 87,906,631 |
| Deletion End | 87,914,909 |
| Deletion Size | 8,296 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,907,030 | 87,907,052 | GAGCCCTGAGTCTCCATTGATAG |
| TUP | 87,907,059 | 87,907,084 | CCAGTTCAGGTTTCTCTGGCTGGACG |
| TUR | 87,907,116 | 87,907,138 | CGCCAACTCTACATCCACCTAAC |
| TDF | 87,914,200 | 87,914,220 | AGGGCACGCTGAAGAGGAGAG |
| TDP | 87,914,230 | 87,914,256 | TGTTGGAGGTAAATGCCAGCATGTGCT |
| TDR | 87,914,272 | 87,914,293 | GAGTTGGGCTGTAACACAGAGG |
PCR Primers
| Primer | Sequence |
| SU | AAACAACCACACGAGCTAGC |
| SD | GGTGGAAACAGCAGCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11596A-A4 | VGB6 | C57BL/6NTac | |
| 11596A-C2 | VGB6 | C57BL/6NTac | |
| 11596A-D7 | VGB6 | C57BL/6NTac | |
| 11596A-E6 | VGB6 | C57BL/6NTac | |
| 11596A-H6 | VGB6 | C57BL/6NTac | |
| 11596A-G6 | VGB6 | C57BL/6NTac | |
