| Gene | Atp5j |
| Chromosome | 16 |
| Deletion Start | 84,831,489 |
| Deletion End | 84,827,937 |
| Deletion Size | 3,553 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,831,459 | 84,831,480 | AGGATCTTCAGGCTCTCCTCTG |
| TUP | 84,831,434 | 84,831,457 | CCTTCGGTCAGCAGTCTCTGTGCA |
| TUR | 84,831,387 | 84,831,408 | GTTCCTTATTAAAGGCCACAGC |
| TDF | 84,828,784 | 84,828,807 | CTGCTTAGTAAGTGTCTTTGATGC |
| TDP | 84,828,752 | 84,828,780 | CAAACCGTGAGGCTTAGAGACTTCCTTGA |
| TDR | 84,828,725 | 84,828,746 | AGAACAGGGTTTCTGAGAGGAG |
PCR Primers
| Primer | Sequence |
| SU | TCTAACAGAACTCTCAGCTAC |
| SD | AAGAGGACAGCACTGCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 339 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11597A-A3 | VGB6 | C57BL/6NTac | |
| 11597A-B9 | VGB6 | C57BL/6NTac | |
| 11597A-G2 | VGB6 | C57BL/6NTac | |
