| Gene | Zpbp |
| Chromosome | 11 |
| Deletion Start | 11,462,361 |
| Deletion End | 11,459,704 |
| Deletion Size | 2,658 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,461,871 | 11,461,890 | ACCTCTGTGGGCTCGTGAAG |
| TUP | 11,461,844 | 11,461,869 | AGGCCCTGCTCCAAGAGATTATCCGA |
| TUR | 11,461,800 | 11,461,820 | GCAATGACCCACGAACTGAAG |
| TDF | 11,460,001 | 11,460,019 | GCCCTGTAAGGTGGGAGAC |
| TDP | 11,459,972 | 11,459,995 | TAAGTCAGCACAGAAAGGCTGGGC |
| TDR | 11,459,903 | 11,459,926 | TGTTACCTACTGCTCTGCATAGTC |
PCR Primers
| Primer | Sequence |
| SU | TCAGGAACTTCATGCATTGG |
| SD | ACCATAGTGAGTAGTGCATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 424 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 117 bp | + | + | - |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11600A-F9 | VGB6 | C57BL/6NTac | |
| 11600C-H12 | VGB6 | C57BL/6NTac | |
| 11600C-E9 | VGB6 | C57BL/6NTac | |
