| Gene | 1110001J03Rik |
| Chromosome | 6 |
| Deletion Start | 38,534,856 |
| Deletion End | 38,539,368 |
| Deletion Size | 4,513 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,535,090 | 38,535,111 | TCAGGACAAACGTGTCTGAGTC |
| TUP | 38,535,114 | 38,535,135 | CCTGTCCTCCACCTGCGGGTCT |
| TUR | 38,535,136 | 38,535,155 | AAGGGCTGGAGTCTGTGGTG |
| TDF | 38,539,202 | 38,539,221 | TCCAAGCTGCCACCTATCTC |
| TDP | 38,539,227 | 38,539,254 | TTTGAGTAGCATCCGGCAACATGTAGCC |
| TDR | 38,539,291 | 38,539,310 | AAACCAGCAGACTCCTCCAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTACGGCAGTCCAAAGG |
| SD | AAAGTTCAGCCAATTTACTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 312 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 589 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11601A-A1 | VGB6 | C57BL/6NTac | |
| 11601A-A4 | VGB6 | C57BL/6NTac | |
| 11601A-A10 | VGB6 | C57BL/6NTac | |
| 11601A-B7 | VGB6 | C57BL/6NTac | |
| 11601A-D7 | VGB6 | C57BL/6NTac | |
| 11601A-D3 | VGB6 | C57BL/6NTac | |
