| Gene | F13a1 |
| Chromosome | 13 |
| Deletion Start | 37,047,483 |
| Deletion End | 37,025,676 |
| Deletion Size | 21,808 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,046,919 | 37,046,937 | GGTGAGAGGTGCCAGATTC |
| TUP | 37,046,892 | 37,046,918 | TGACAGTAACAGAGATGGAAGCCCTCA |
| TUR | 37,046,855 | 37,046,874 | TGCCTTGCCTTGTCCCTTAG |
| TDF | 37,026,186 | 37,026,206 | GTCTCACATAGGGAGGGACAG |
| TDP | 37,026,164 | 37,026,185 | TCGCAACAGGAAGAGCCCGACT |
| TDR | 37,026,115 | 37,026,139 | GGTCAGAGTTCAAGTGTTCAGATTC |
PCR Primers
| Primer | Sequence |
| SU | ATATGCAGGCTTTGAGGTGC |
| SD | ACAATGGTAAGATGCGAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 444 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11602B-B6 | VGB6 | C57BL/6NTac | |
| 11602B-H8 | VGB6 | C57BL/6NTac | |
