| Gene | Zfp36 |
| Chromosome | 7 |
| Deletion Start | 28,379,194 |
| Deletion End | 28,377,540 |
| Deletion Size | 1,655 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,378,465 | 28,378,485 | GTCGATGAGCCATGACCTGTC |
| TUP | 28,378,440 | 28,378,462 | CCGACCACGGAGGAACCGAATCC |
| TUR | 28,378,402 | 28,378,425 | GGGATGGAGTCCGAGTTTATGTTC |
| TDF | 28,378,250 | 28,378,271 | CCTACTTCGCCTACTGCAACTC |
| TDP | 28,378,223 | 28,378,249 | CCACCACCTCCTCTCGATACAAGACCG |
| TDR | 28,378,189 | 28,378,206 | CAACGCCCGCTTTCTGAG |
PCR Primers
| Primer | Sequence |
| SU | CACCTCCAGTTGGTCGTAC |
| SD | GAAGACCAACTGTCAACTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11603A-B8 | VGB6 | C57BL/6NTac | |
| 11603B-G9 | VGB6 | C57BL/6NTac | |
