| Gene | Cav3 |
| Chromosome | 6 |
| Deletion Start | 112,459,797 |
| Deletion End | 112,472,513 |
| Deletion Size | 12,717 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,460,165 | 112,460,188 | GGAGTCACTGGGAAGATACAGAAC |
| TUP | 112,460,190 | 112,460,213 | TGGGCCAAGTGGTTGCTTTGCTTT |
| TUR | 112,460,232 | 112,460,251 | CCCAAGCCCACTATGGAGAC |
| TDF | 112,472,294 | 112,472,315 | TGTTCGCCTGTATCTCCTTCTG |
| TDP | 112,472,318 | 112,472,338 | ACATCTGGGCCGTGGTGCCCT |
| TDR | 112,472,364 | 112,472,382 | GATGTGGCTGATGCACTGG |
PCR Primers
| Primer | Sequence |
| SU | CACCAGCAGCTTTAACTAGAG |
| SD | CAAAGCCTTGCGCTAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 308 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11604B-F10 | VGB6 | C57BL/6NTac | |
| 11604B-H5 | VGB6 | C57BL/6NTac | |
