| Gene | Lor |
| Chromosome | 3 |
| Deletion Start | 92,081,974 |
| Deletion End | 92,080,488 |
| Deletion Size | 1,487 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,081,822 | 92,081,841 | GCTAGAGCCTCCTCCAGATG |
| TUP | 92,081,842 | 92,081,861 | AGCCGCCTCCGCAGCCAGAG |
| TUR | 92,081,922 | 92,081,942 | TCCTGTGGGTTGTGGAAAGAC |
| TDF | 92,080,902 | 92,080,923 | ATTACTCCTCTCAGCAGACCAG |
| TDP | 92,080,865 | 92,080,885 | CCCCAGCAGAGCTACGGAGGG |
| TDR | 92,080,827 | 92,080,845 | GAGCCACCTCCACAGCTAC |
PCR Primers
| Primer | Sequence |
| SU | CACATAGTAGAAGAAAGGCAG |
| SD | ATTGACTGAGGAGACACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11607A-A10 | VGB6 | C57BL/6NTac | |
| 11607A-B6 | VGB6 | C57BL/6NTac | |
| 11607A-G9 | VGB6 | C57BL/6NTac | |
