| Gene | Dbh |
| Chromosome | 2 |
| Deletion Start | 27,165,519 |
| Deletion End | 27,182,809 |
| Deletion Size | 17,291 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,166,221 | 27,166,241 | AGCAACTCCATGCCACACTAC |
| TUP | 27,166,247 | 27,166,268 | CATGTGCACCTGTTGTGGGCCA |
| TUR | 27,166,321 | 27,166,339 | AGCACAGAAGGCGTGTTTC |
| TDF | 27,182,319 | 27,182,338 | GCACCGCCGGATATGGTTAG |
| TDP | 27,182,348 | 27,182,372 | TTTGCAGACGCCTTGTTGGACAGTG |
| TDR | 27,182,398 | 27,182,418 | TCCGAGGAAAGGATCAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGTAGCTGTTTCCAACAGGC |
| SD | TGAATCTGGACTACCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 354 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11608A-C6 | VGB6 | C57BL/6NTac | |
| 11608A-C10 | VGB6 | C57BL/6NTac | |
