| Gene | Atf5 |
| Chromosome | 7 |
| Deletion Start | 44,892,967 |
| Deletion End | 44,890,627 |
| Deletion Size | 2,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,814,233 | 44,814,252 | GCAGTCAAGCAGCCACAGAG |
| TUP | 44,814,207 | 44,814,232 | CAGGATTACAGACGTGGGAGCAGACC |
| TUR | 44,814,168 | 44,814,191 | CAGTGAAGCAGTCACCCAACTAAC |
| TDF | 44,813,605 | 44,813,623 | GGCTGGCTGGTCACTTGTC |
| TDP | 44,813,571 | 44,813,600 | TTAATGAATGGGACATGAGTGAACACCGAA |
| TDR | 44,813,545 | 44,813,567 | GTCCCTGAGGACTGTGCTTTATC |
PCR Primers
| Primer | Sequence |
| SU | GGAGTTTCCAATAATGTCCC |
| SD | TGGCTTCCTGCTTTATGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 77712 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 78194 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11612A-E2 | VGB6 | C57BL/6NTac | |
| 11612A-F12 | VGB6 | C57BL/6NTac | |
