| Gene | Pcsk1n |
| Chromosome | X |
| Deletion Start | 7,919,917 |
| Deletion End | 7,923,106 |
| Deletion Size | 3,190 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,920,132 | 7,920,151 | TAGGGCTTGGCTAGGGTGAC |
| TUP | 7,920,153 | 7,920,171 | TGGCCTGCAGCTGACAGCC |
| TUR | 7,920,205 | 7,920,224 | GCGCCCTGAACAACATCATC |
| TDF | 7,922,863 | 7,922,884 | CAGCGATGGCAAATACATCCTG |
| TDP | 7,922,886 | 7,922,908 | CCGCATTCCGCAATCCTTGCAGG |
| TDR | 7,922,927 | 7,922,945 | GCTCCGAACTTCCGGTGAG |
PCR Primers
| Primer | Sequence |
| SU | TGCAGACTTCTGTTCTCCAC |
| SD | TACTGTGCTGTCCTCAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11614A-A7 | VGB6 | C57BL/6NTac | |
| 11614A-B5 | VGB6 | C57BL/6NTac | |
| 11614A-H7 | VGB6 | C57BL/6NTac | |
