| Gene | Srl |
| Chromosome | 16 |
| Deletion Start | 4,523,050 |
| Deletion End | 4,493,783 |
| Deletion Size | 29,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,522,521 | 4,522,542 | TGGAACTGGGATAGGGTAGAAC |
| TUP | 4,522,492 | 4,522,520 | TGAATGGAAAGCTGAGAACTGGTGACTGT |
| TUR | 4,522,442 | 4,522,461 | CGGAAGGCTCTCCTCCATTC |
| TDF | 4,494,517 | 4,494,537 | TGCTTGGCTTACAGGTTACAG |
| TDP | 4,494,490 | 4,494,515 | CCATCTACTTGAGGGAAGCTGAGGCA |
| TDR | 4,494,443 | 4,494,463 | CCACTGAGGGTGGTGTCATTC |
PCR Primers
| Primer | Sequence |
| SU | TTGTAGGCATTTCCAGGTCC |
| SD | AATCCAAAGCAGTTAGGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 486 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11615A-B11 | VGB6 | C57BL/6NTac | |
| 11615A-C4 | VGB6 | C57BL/6NTac | |
| 11615A-D12 | VGB6 | C57BL/6NTac | |
| 11615A-E8 | VGB6 | C57BL/6NTac | |
| 11615A-F7 | VGB6 | C57BL/6NTac | |
| 11615A-F10 | VGB6 | C57BL/6NTac | |
