| Gene | Slc25a5 |
| Chromosome | X |
| Deletion Start | 36,795,734 |
| Deletion End | 36,798,600 |
| Deletion Size | 2,867 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,796,072 | 36,796,092 | CGGCACAGAGAACTAGGATGG |
| TUP | 36,796,095 | 36,796,117 | TTGCCCGAGAGGCTCTGTCTAGC |
| TUR | 36,796,147 | 36,796,164 | TTTCCAGCGCCTGCTTTC |
| TDF | 36,797,984 | 36,798,005 | CAGCTCGGTGGTAGAGCATTAG |
| TDP | 36,798,006 | 36,798,035 | CCCACCGTACTCAAGGCCCTATATTCAATC |
| TDR | 36,798,048 | 36,798,070 | CCCTTCAGAGCCTACAGCATTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTACTCTGGCCTGATCAG |
| SD | AACAAACAACGAACAAAACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 415 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11616A-C7 | VGB6 | C57BL/6NTac | |
| 11616A-H6 | VGB6 | C57BL/6NTac | |
