| Gene | Grhpr |
| Chromosome | 4 |
| Deletion Start | 44,981,455 |
| Deletion End | 44,990,509 |
| Deletion Size | 9,055 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,982,154 | 44,982,173 | GGCCTGGCCCTTATGCTTAG |
| TUP | 44,982,179 | 44,982,201 | ATCCGCTGCAGGCGGGTCACTTT |
| TUR | 44,982,212 | 44,982,235 | CCAAACTGGCATGGTAACACAAAG |
| TDF | 44,989,772 | 44,989,793 | TGTCCTTGCTGCCTCTTTAGTG |
| TDP | 44,989,794 | 44,989,821 | AAGGCACCCATTATTGCAGTCCTTAGCA |
| TDR | 44,989,825 | 44,989,843 | GCCACAGCGATCAGGTCAG |
PCR Primers
| Primer | Sequence |
| SU | GCTCTCCAAGTCCTACACTC |
| SD | CAGATGCCGAGTCAGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 347 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11618A-B5 | VGB6 | C57BL/6NTac | |
| 11618A-H8 | VGB6 | C57BL/6NTac | |
| 11618A-F12 | VGB6 | C57BL/6NTac | |
| 11618A-A3 | VGB6 | C57BL/6NTac | |
| 11618A-G9 | VGB6 | C57BL/6NTac | |
