| Gene | Emg1 |
| Chromosome | 6 |
| Deletion Start | 124,712,040 |
| Deletion End | 124,704,510 |
| Deletion Size | 7,531 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,711,970 | 124,711,991 | AGGAGCAGGACTGGGAGACTAC |
| TUP | 124,711,946 | 124,711,968 | CCGCCTAAGAAGCTCCGGCTTGG |
| TUR | 124,711,897 | 124,711,917 | CCCTTCCAGCACCACAATGAG |
| TDF | 124,705,184 | 124,705,203 | CAAACTGAGCGTCCGAGCAG |
| TDP | 124,705,160 | 124,705,183 | CCGACGGCCCTCAGAAGCTATTGA |
| TDR | 124,705,115 | 124,705,136 | CAGAACTGGCCCAGAACCTTAC |
PCR Primers
| Primer | Sequence |
| SU | AACTCCGCGCACAGGAATG |
| SD | ACGCCTACTGTCAAAATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11620A-C10 | VGB6 | C57BL/6NTac | |
| 11620A-D2 | VGB6 | C57BL/6NTac | |
