| Gene | Arl16 |
| Chromosome | 11 |
| Deletion Start | 120,467,420 |
| Deletion End | 120,465,649 |
| Deletion Size | 1,772 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,466,853 | 120,466,874 | GTCACTGGCTGTGGATGCTTAG |
| TUP | 120,466,832 | 120,466,851 | TCCCTCATGGCGGTGCTGCC |
| TUR | 120,466,809 | 120,466,828 | TGTGCTGTGGTGAGCTTCTG |
| TDF | 120,465,943 | 120,465,964 | GCAGGATGAAGGGCAAGGTATC |
| TDP | 120,465,907 | 120,465,933 | TGCTCTACTTGAGTACAGCCAAGACCA |
| TDR | 120,465,854 | 120,465,875 | GCTCGCCACAAAGTTGTTCTAC |
PCR Primers
| Primer | Sequence |
| SU | ACTTCCGCTTCCGCCTTTC |
| SD | CTCCGTGCAGTCCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11621A-C2 | VGB6 | C57BL/6NTac | |
| 11621A-C5 | VGB6 | C57BL/6NTac | |
| 11621A-E11 | VGB6 | C57BL/6NTac | |
| 11621A-H6 | VGB6 | C57BL/6NTac | |
