| Gene | 0610037L13Rik |
| Chromosome | 4 |
| Deletion Start | 107,889,989 |
| Deletion End | 107,896,959 |
| Deletion Size | 6,971 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,890,771 | 107,890,789 | GCCCTAGAGGCCCAAACAG |
| TUP | 107,890,794 | 107,890,817 | ACACCCTTCATCGTCCCAGGCTGT |
| TUR | 107,890,838 | 107,890,857 | TGGGTATGTGAGGCACTGAG |
| TDF | 107,896,151 | 107,896,171 | TGCTGACTGCAATTCCTCCAG |
| TDP | 107,896,173 | 107,896,195 | ATGGTCACCACCAGGCGATCCCA |
| TDR | 107,896,253 | 107,896,274 | TGTGACGTGGGCCTAAATGAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCAGCTGTCGCAGCGAC |
| SD | GGATCAGGTACAGAATAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 268 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11622A-B4 | VGB6 | C57BL/6NTac | |
| 11622A-D9 | VGB6 | C57BL/6NTac | |
| 11622A-C2 | VGB6 | C57BL/6NTac | |
