| Gene | C030048B08Rik |
| Chromosome | 5 |
| Deletion Start | 3,596,073 |
| Deletion End | 3,584,559 |
| Deletion Size | 11,515 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,595,908 | 3,595,930 | GGACACTAAATTCCTGTGAGCAC |
| TUP | 3,595,887 | 3,595,907 | TAGCTCCCCAAGGCCTGCCTC |
| TUR | 3,595,866 | 3,595,886 | CATCCCTGCCATGCCTTTATG |
| TDF | 3,585,442 | 3,585,465 | CAGGGTGGAGATAGGTGTATAGTG |
| TDP | 3,585,410 | 3,585,439 | TGGAACATGCCAAATTCCAGAAACTGAGCC |
| TDR | 3,585,383 | 3,585,406 | CCGTAAACTTGGGTTTGACCATTC |
PCR Primers
| Primer | Sequence |
| SU | CTCGTCAGGTGAGAGCGTC |
| SD | ACACAGATTGCTCAATACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 363 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11624A-C8 | VGB6 | C57BL/6NTac | |
| 11624A-H5 | VGB6 | C57BL/6NTac | |
| 11624A-D4 | VGB6 | C57BL/6NTac | |
| 11624A-H9 | VGB6 | C57BL/6NTac | |
| 11624A-D1 | VGB6 | C57BL/6NTac | |
