| Gene | Clcn2 |
| Chromosome | 16 |
| Deletion Start | 20,715,992 |
| Deletion End | 20,703,219 |
| Deletion Size | 12,774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,715,904 | 20,715,922 | ACGAGCAGACCCTGGTAAG |
| TUP | 20,715,880 | 20,715,903 | TGAAACCCAAGGGTTGCCCTACGG |
| TUR | 20,715,835 | 20,715,856 | GGCCATCTGTCCTCACACTTTC |
| TDF | 20,703,621 | 20,703,644 | CAGACCCACACCATCTTCTCATTG |
| TDP | 20,703,594 | 20,703,619 | TGGGAGTGGACCATGCTTATGTCACC |
| TDR | 20,703,541 | 20,703,563 | CCATGTCACCTCACCTCCTTTAG |
PCR Primers
| Primer | Sequence |
| SU | TGCGAGATCGTGTTTCAAGC |
| SD | ACATGAGTGGCTAATTAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 435 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11626A-C11 | VGB6 | C57BL/6NTac | |
| 11626A-D9 | VGB6 | C57BL/6NTac | |
