| Gene | Eef1d |
| Chromosome | 15 |
| Deletion Start | 75,903,805 |
| Deletion End | 75,895,527 |
| Deletion Size | 8,279 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,903,441 | 75,903,459 | TCTCGGCTGACCACGTATG |
| TUP | 75,903,414 | 75,903,437 | AGACAAGCCACTCTTCGACCAGGC |
| TUR | 75,903,392 | 75,903,412 | CCTCTGACGGTAGGAGCTTTC |
| TDF | 75,896,300 | 75,896,318 | ACGCCAGTACGCAGAGAAG |
| TDP | 75,896,275 | 75,896,299 | AAGGCCAAGAAGCCCACACTGGTGG |
| TDR | 75,896,233 | 75,896,254 | CGCCACTCACAGGTTTAACATC |
PCR Primers
| Primer | Sequence |
| SU | ACTAAGGTGTGGATGTGTGC |
| SD | GAGGATGTATCCCTCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11628A-G12 | VGB6 | C57BL/6NTac | |
| 11628B-B12 | VGB6 | C57BL/6NTac | |
