| Gene | 2610002J02Rik |
| Chromosome | 4 |
| Deletion Start | 155,250,458 |
| Deletion End | 155,256,216 |
| Deletion Size | 5,759 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,250,764 | 155,250,781 | ACCTGACCCAGAGTCCAC |
| TUP | 155,250,782 | 155,250,804 | TGTGCCTCCTGAGGCCTTCACTG |
| TUR | 155,250,815 | 155,250,832 | GGCGTCCAGGAGAAAGTC |
| TDF | 155,256,103 | 155,256,121 | TGTGCCTGGTGACAGTTTC |
| TDP | 155,256,127 | 155,256,152 | CCCTGTAGGCTGACCCAGCTAGATGT |
| TDR | 155,256,154 | 155,256,175 | AGCACTGAGCAAGGTGGCTATC |
PCR Primers
| Primer | Sequence |
| SU | GTCGCCCATTTATCCACTTC |
| SD | AGTCCACAGAGGATCAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 243 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11629A-A2 | VGB6 | C57BL/6NTac | |
| 11629A-C2 | VGB6 | C57BL/6NTac | |
| 11629A-E1 | VGB6 | C57BL/6NTac | |
| 11629A-F9 | VGB6 | C57BL/6NTac | |
| 11629A-G6 | VGB6 | C57BL/6NTac | |
| 11629A-H2 | VGB6 | C57BL/6NTac | |
