| Gene | A430005L14Rik |
| Chromosome | 4 |
| Deletion Start | 153,957,311 |
| Deletion End | 153,961,605 |
| Deletion Size | 4,295 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,957,648 | 153,957,668 | TAGGGTGACTGGGACCTAAAC |
| TUP | 153,957,669 | 153,957,690 | CCCGGCTGCAAAGGGTCTCTGG |
| TUR | 153,957,711 | 153,957,730 | TCAGGCTAAGGGACTCACTG |
| TDF | 153,961,303 | 153,961,326 | GACCATGTCTGTTGTCATCTCTTG |
| TDP | 153,961,337 | 153,961,364 | AGCATCTTTCTTACCAAGGGACCTGTGC |
| TDR | 153,961,430 | 153,961,449 | TTGCCAGGGCATCCACATTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCAAACATGGCGGCGAC |
| SD | AAATGGTTAGCGCCACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 450 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11634A-B10 | VGB6 | C57BL/6NTac | |
| 11634A-B12 | VGB6 | C57BL/6NTac | |
| 11634A-D8 | VGB6 | C57BL/6NTac | |
| 11634A-E6 | VGB6 | C57BL/6NTac | |
| 11634A-F2 | VGB6 | C57BL/6NTac | |
| 11634A-G12 | VGB6 | C57BL/6NTac | |
