| Gene | Nudt1 |
| Chromosome | 5 |
| Deletion Start | 140,334,453 |
| Deletion End | 140,337,841 |
| Deletion Size | 3,389 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,335,318 | 140,335,339 | TGCTGGAAGAAAGTGGTCTGAG |
| TUP | 140,335,342 | 140,335,366 | TGGATACACTGCACAAGGTAGGCCA |
| TUR | 140,335,367 | 140,335,390 | GAGCCCACAAATTCAAACGAGATG |
| TDF | 140,337,717 | 140,337,738 | TCAGGACACGATCCTCAGTTAC |
| TDP | 140,337,740 | 140,337,764 | CGCTGCGAGAGGTGGACTCATTCTA |
| TDR | 140,337,770 | 140,337,788 | CCAGAACCAGCCAGACTTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTCAAAGGCACATTTGTAC |
| SD | AGGATCTCTCTCCTATCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 323 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 438 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11639A-F1 | VGB6 | C57BL/6NTac | |
| 11639A-F4 | VGB6 | C57BL/6NTac | |
