| Gene | Kdm4d |
| Chromosome | 9 |
| Deletion Start | 14,464,557 |
| Deletion End | 14,462,987 |
| Deletion Size | 1,571 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,464,124 | 14,464,143 | TCCCTGTTTGATGGGAAGAC |
| TUP | 14,464,098 | 14,464,123 | TCAACAGTGGAATGTGGGTCACCTGG |
| TUR | 14,464,041 | 14,464,063 | TGTTGACGCCCTCAATCACTATG |
| TDF | 14,463,319 | 14,463,342 | GTGAAAGGTGATGATGTCCAGAAG |
| TDP | 14,463,295 | 14,463,316 | TGACTCAGCCAGAGCCTCACCA |
| TDR | 14,463,265 | 14,463,284 | GTGACCAGAAGAAGGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCCAACGGAACATATTAGACC |
| SD | TAGAGTTGGCCTTGAGCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11643A-E11 | VGB6 | C57BL/6NTac | |
| 11643A-D10 | VGB6 | C57BL/6NTac | |
| 11643A-C6 | VGB6 | C57BL/6NTac | |
| 11643A-H2 | VGB6 | C57BL/6NTac | |
