| Gene | Kcnmb1 |
| Chromosome | 11 |
| Deletion Start | 33,964,686 |
| Deletion End | 33,970,381 |
| Deletion Size | 5,696 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,964,845 | 33,964,867 | CTCCACTTCCTGGATTGACCTAC |
| TUP | 33,964,872 | 33,964,894 | CCCTTGACCCGTCACGGTAGTCA |
| TUR | 33,964,907 | 33,964,929 | CCTGAGCCTGAAGATGACGAATG |
| TDF | 33,969,608 | 33,969,626 | TGCTGCATGGTGTGGTATC |
| TDP | 33,969,627 | 33,969,651 | TCTTCTCCAGAGGGAGTCACGGTGA |
| TDR | 33,969,659 | 33,969,676 | GGCATGTGCCTTGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGTTACCAGAGGCCAGAG |
| SD | AGCGTCATTGCAGGTAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11644A-A11 | VGB6 | C57BL/6NTac | |
| 11644A-B5 | VGB6 | C57BL/6NTac | |
| 11644A-G3 | VGB6 | C57BL/6NTac | |
