| Gene | P2rx1 |
| Chromosome | 11 |
| Deletion Start | 72,999,301 |
| Deletion End | 73,014,130 |
| Deletion Size | 14,830 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,999,939 | 72,999,959 | TCCATTCCCAAGTGTGACATC |
| TUP | 72,999,960 | 72,999,981 | TCCTTCCCTGGCACAGGGTCAG |
| TUR | 72,999,984 | 73,000,003 | GCTGAAGGAGACCTGTGCTG |
| TDF | 73,013,678 | 73,013,699 | CCTTAGCCTGTCTTCAGGAAAC |
| TDP | 73,013,705 | 73,013,729 | TCCAGAGTGGCAGGATTTAGCCAGG |
| TDR | 73,013,780 | 73,013,800 | GGGACGGAGAATGGGAATCTG |
PCR Primers
| Primer | Sequence |
| SU | AGCAGTCAAAAGCTGCTCTG |
| SD | TACTGGTGTGTGAGGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11645B-A3 | VGB6 | C57BL/6NTac | |
| 11645B-C2 | VGB6 | C57BL/6NTac | |
| 11645B-F4 | VGB6 | C57BL/6NTac | |
