| Gene | Cbx4 |
| Chromosome | 11 |
| Deletion Start | 119,086,014 |
| Deletion End | 119,080,794 |
| Deletion Size | 5,221 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,085,442 | 119,085,460 | GCGCAGGCACTGACTGATG |
| TUP | 119,085,417 | 119,085,441 | TGCAGCAGAAAATGGCTCCTTTCCC |
| TUR | 119,085,398 | 119,085,416 | CCGTTCGGTGGAGGGAAAG |
| TDF | 119,080,885 | 119,080,904 | CGTGACGGTGTAGTGGGAAG |
| TDP | 119,080,861 | 119,080,883 | AGTCGCGCCCACTAGGACTCAGC |
| TDR | 119,080,836 | 119,080,858 | TTTCTCCTTGGGAACCTCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGATTTGCATGAGTTCTCGT |
| SD | GGATAGTCGGGCCTTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11646A-B7 | VGB6 | C57BL/6NTac | |
| 11646A-D5 | VGB6 | C57BL/6NTac | |
| 11646A-H8 | VGB6 | C57BL/6NTac | |
