| Gene | Abcb9 |
| Chromosome | 5 |
| Deletion Start | 124,090,173 |
| Deletion End | 124,062,603 |
| Deletion Size | 27,571 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,089,581 | 124,089,601 | CGTAGCAGCTCTGGGTAAGTG |
| TUP | 124,089,554 | 124,089,577 | CCTTGGGTCTGACCACACTGAGCC |
| TUR | 124,089,522 | 124,089,541 | AGGCACCCTGCAAGTACATC |
| TDF | 124,063,085 | 124,063,105 | TGGGCTGTCTGGAATAGAAAC |
| TDP | 124,063,061 | 124,063,083 | CAGGACTTGAGCTGCCTTGGTCC |
| TDR | 124,063,030 | 124,063,052 | GCTGACATTCAGTTCTTGGGTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGTCTGGCTGGCTTCTG |
| SD | CTCTCCCAGACATCCTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 600 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 387 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11648B-F7 | VGB6 | C57BL/6NTac | |
| 11648C-A3 | VGB6 | C57BL/6NTac | |
| 11648C-H4 | VGB6 | C57BL/6NTac | |
