| Gene | Gm128 |
| Chromosome | 3 |
| Deletion Start | 95,240,978 |
| Deletion End | 95,237,178 |
| Deletion Size | 3,801 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,240,199 | 95,240,217 | GCCTTGCACCTACCAGTTG |
| TUP | 95,240,179 | 95,240,198 | TGCGCTTGCAACAGGCACCT |
| TUR | 95,240,156 | 95,240,174 | TCGAGTCCAGAGGGCATTC |
| TDF | 95,237,721 | 95,237,740 | AGTATGCGTGAGGTCCTGAG |
| TDP | 95,237,697 | 95,237,719 | TTGACTTGCAACACAGCTGGGCA |
| TDR | 95,237,661 | 95,237,681 | GGCTCCACCCACCAATCTATG |
PCR Primers
| Primer | Sequence |
| SU | AGATTTAAGTCTGTGCCTAGG |
| SD | TGCTGGCTCTAATGTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 412 bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11650A-F4 | VGB6 | C57BL/6NTac | |
