| Gene | Ctss |
| Chromosome | 3 |
| Deletion Start | 95,526,903 |
| Deletion End | 95,556,199 |
| Deletion Size | 29,297 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,527,738 | 95,527,759 | GGTTGAGCTGCTTGGTTGTTTG |
| TUP | 95,527,765 | 95,527,788 | CTTTGGTGTTCACGCTCAGGCCAA |
| TUR | 95,527,839 | 95,527,859 | TGTCCAGGCAACACAGAGAAG |
| TDF | 95,555,531 | 95,555,550 | GCTGGGCCACACCTGTAATC |
| TDP | 95,555,552 | 95,555,579 | CAGGCTTGGAAAGTCAAAGCTGGAGAAC |
| TDR | 95,555,584 | 95,555,604 | ACGCAGTCTGGCTGCAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGTCCTGTTCTCTGCTGAG |
| SD | AATGTTATCTAAGGACGACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 403 bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11653A-B12 | VGB6 | C57BL/6NTac | |
| 11653B-A5 | VGB6 | C57BL/6NTac | |
| 11653C-C9 | VGB6 | C57BL/6NTac | |
| 11653B-A2 | VGB6 | C57BL/6NTac | |
