| Gene | Tmub1 |
| Chromosome | 5 |
| Deletion Start | 24,446,916 |
| Deletion End | 24,445,890 |
| Deletion Size | 1,027 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,446,688 | 24,446,706 | CCCAGCCTGAGACACAGAG |
| TUP | 24,446,665 | 24,446,686 | TCCATCTGCACAGCCAGAGCCT |
| TUR | 24,446,572 | 24,446,593 | GGCTACCTGCTCAGAGTCATTG |
| TDF | 24,446,260 | 24,446,279 | CCTCAGGACCCAGTTTCCAG |
| TDP | 24,446,236 | 24,446,258 | CCAGGAACAGCAGGTTCGGCTCA |
| TDR | 24,446,188 | 24,446,209 | GACTGCCTAGTGTCTGAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCACTTGGAATCTGGTGATG |
| SD | AAGTGCCTGATCCAGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 315 bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11654C-B5 | VGB6 | C57BL/6NTac | |
| 11654C-G6 | VGB6 | C57BL/6NTac | |
| 11654C-D4 | VGB6 | C57BL/6NTac | |
| 11654C-B11 | VGB6 | C57BL/6NTac | |
| 11654C-F12 | VGB6 | C57BL/6NTac | |
| 11654A-D9 | VGB6 | C57BL/6NTac | |
| 11654A-E11 | VGB6 | C57BL/6NTac | |
