| Gene | Hsp90aa1 |
| Chromosome | 12 |
| Deletion Start | 110,695,677 |
| Deletion End | 110,691,519 |
| Deletion Size | 4,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,694,758 | 110,694,780 | GGGAAGGTTAGCTGCTAGGAAAC |
| TUP | 110,694,734 | 110,694,756 | TCCAGCAAGCATGGAGGCTTTGA |
| TUR | 110,694,685 | 110,694,704 | TCCACGACCCATTGGTTCAC |
| TDF | 110,691,852 | 110,691,874 | GCCTCAGCTAAAGGGTTTGAATG |
| TDP | 110,691,819 | 110,691,848 | TGAACAGTGACCTTTGTGTTTGAGGATGGC |
| TDR | 110,691,790 | 110,691,809 | CACTGGGACAGAGGCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACATGCGCTTCGTAATTACC |
| SD | AGCTAAACACTTCAGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 312 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11657A-A11 | VGB6 | C57BL/6NTac | |
| 11657A-B1 | VGB6 | C57BL/6NTac | |
| 11657A-F12 | VGB6 | C57BL/6NTac | |
| 11657A-C3 | VGB6 | C57BL/6NTac | |
| 11657A-F4 | VGB6 | C57BL/6NTac | |
| 11657A-D6 | VGB6 | C57BL/6NTac | |
| 11657A-C5 | VGB6 | C57BL/6NTac | |
