| Gene | Cyp8b1 |
| Chromosome | 9 |
| Deletion Start | 121,916,261 |
| Deletion End | 121,914,713 |
| Deletion Size | 1,549 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,915,853 | 121,915,870 | GGCAAGGCTTGGAGGAAC |
| TUP | 121,915,829 | 121,915,850 | AACCAGGCCATGCTGGACAGCC |
| TUR | 121,915,786 | 121,915,807 | GCACCCAGACTCGAACCTTTAG |
| TDF | 121,915,334 | 121,915,353 | GAAGGCTGTGCGTGAGGAAG |
| TDP | 121,915,310 | 121,915,332 | CACCAGGGTCATGGGTAAGGCCA |
| TDR | 121,915,266 | 121,915,285 | CAGGGCACTGGGAGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CATCGTCTCTCTAGTCAGC |
| SD | CATCGAGTAAGGCAGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11658A-D5 | VGB6 | C57BL/6NTac | |
| 11658A-F7 | VGB6 | C57BL/6NTac | |
| 11658A-H3 | VGB6 | C57BL/6NTac | |
| 11658A-G9 | VGB6 | C57BL/6NTac | |
