| Gene | 0610037P05Rik |
| Chromosome | 16 |
| Deletion Start | 14,317,318 |
| Deletion End | 14,300,107 |
| Deletion Size | 17,212 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,317,088 | 14,317,110 | GAGGAAGGGACTGGTATCTGTTG |
| TUP | 14,317,058 | 14,317,085 | CCCTCAGGACCTCTGGGTATCTCTTAGA |
| TUR | 14,317,037 | 14,317,055 | GCGCTGTCTGCTCAGTAGG |
| TDF | 14,300,776 | 14,300,796 | GGGCTCAGCTAACAGGCAATC |
| TDP | 14,300,741 | 14,300,767 | TTTGTTGGCCCTACAATGCTGATTGCA |
| TDR | 14,300,718 | 14,300,738 | GGACTCAGGAGGAAGGACAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTTTTCTGCAGTGTAGCC |
| SD | GTGGCATCCAGCAAGCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 301 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 255 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11660A-E10 | VGB6 | C57BL/6NTac | |
| 11660B-D3 | VGB6 | C57BL/6NTac | |
| 11660B-D9 | VGB6 | C57BL/6NTac | |
