| Gene | Msto1 |
| Chromosome | 3 |
| Deletion Start | 88,913,873 |
| Deletion End | 88,909,678 |
| Deletion Size | 4,196 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,913,618 | 88,913,635 | ACAGAACAGGCCGGACAC |
| TUP | 88,913,592 | 88,913,617 | TGCATGGCCAGGAAACCTATACGCCT |
| TUR | 88,913,553 | 88,913,573 | CACCATCGCCTCACCTTTCAG |
| TDF | 88,910,643 | 88,910,663 | GTGGTGCTCAGAGGCATAGAC |
| TDP | 88,910,615 | 88,910,640 | AGCTAGCCACACCAGGTAAAGACTGC |
| TDR | 88,910,561 | 88,910,581 | GCGTGCTAGGGAAACAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGAAGCAGGTGAGAGAAC |
| SD | TGAGGACAGAGCAAGAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11662A-C1 | VGB6 | C57BL/6NTac | |
| 11662A-C12 | VGB6 | C57BL/6NTac | |
| 11662A-D12 | VGB6 | C57BL/6NTac | |
| 11662A-A9 | VGB6 | C57BL/6NTac | |
| 11662A-H10 | VGB6 | C57BL/6NTac | |
| 11662A-B3 | VGB6 | C57BL/6NTac | |
