| Gene | Ddost |
| Chromosome | 4 |
| Deletion Start | 138,304,865 |
| Deletion End | 138,312,018 |
| Deletion Size | 7,154 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,304,974 | 138,304,992 | CCTCGTGCTGCTGGACAAC |
| TUP | 138,304,994 | 138,305,014 | TGAACGTGCGGGACACGCACT |
| TUR | 138,305,017 | 138,305,036 | TTCAGGCTGCGGAAGAACAG |
| TDF | 138,311,706 | 138,311,724 | ACAACCGGCTAGGCTACAC |
| TDP | 138,311,730 | 138,311,757 | TGTACTCTTCCACCCAGGTAAGTGAGGG |
| TDR | 138,311,775 | 138,311,794 | CCTAAGGGCGACACCTACTG |
PCR Primers
| Primer | Sequence |
| SU | GAGCCTCAAGTGCGTTTCG |
| SD | TCCATGAGGAGAGATGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 383 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11663A-A8 | VGB6 | C57BL/6NTac | |
| 11663A-B7 | VGB6 | C57BL/6NTac | |
| 11663B-C8 | VGB6 | C57BL/6NTac | |
| 11663B-D2 | VGB6 | C57BL/6NTac | |
| 11663B-F3 | VGB6 | C57BL/6NTac | |
| 11663B-H12 | VGB6 | C57BL/6NTac | |
| 11663B-F12 | VGB6 | C57BL/6NTac | |
| 11663B-E11 | VGB6 | C57BL/6NTac | |
