| Gene | Afp |
| Chromosome | 5 |
| Deletion Start | 90,490,784 |
| Deletion End | 90,508,496 |
| Deletion Size | 17,713 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,491,577 | 90,491,598 | GTCTGGACAGGTGGTTCCTAAC |
| TUP | 90,491,603 | 90,491,630 | TGGCAGTTCTAAGACTTCATGGCTTGGG |
| TUR | 90,491,640 | 90,491,659 | CCGGGAAAGCAATCGTTGTG |
| TDF | 90,507,631 | 90,507,650 | GTGCTTCAGGCTGAGAAGTG |
| TDP | 90,507,651 | 90,507,674 | TAAGGGCTAACCTTGGCACGGAGC |
| TDR | 90,507,730 | 90,507,748 | AGCCGCACCTTCCCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTTTAGATGGTGAAGAAC |
| SD | TAAGCCAAAAGGCTCACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 407 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | 376 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11665A-D11 | VGB6 | C57BL/6NTac | |
| 11665A-E10 | VGB6 | C57BL/6NTac | |
| 11665A-F3 | VGB6 | C57BL/6NTac | |
