| Gene | Gnpda2 |
| Chromosome | 5 |
| Deletion Start | 69,589,781 |
| Deletion End | 69,575,682 |
| Deletion Size | 14,100 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,589,374 | 69,589,394 | TTCTAGGACACCGCCTTCATC |
| TUP | 69,589,355 | 69,589,373 | AGTCCTGATTTCTTACAGC |
| TUR | 69,589,315 | 69,589,337 | GCAATGCGAAACTGAGTCATCAC |
| TDF | 69,576,158 | 69,576,176 | CTCTCCAGGGCTCTGCAAG |
| TDP | 69,576,134 | 69,576,156 | CAAGCACTCAGGCTGCACTGGGA |
| TDR | 69,576,101 | 69,576,125 | GAGTAGACGTGGACTGAGTAGATAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGACAAGTCATGTGAGTCC |
| SD | ACTGCAGAAAGATGACTAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 452 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 364 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11668A-G2 | VGB6 | C57BL/6NTac | |
| 11668D-D1 | VGB6 | C57BL/6NTac | |
| 11668D-G4 | VGB6 | C57BL/6NTac | |
