11669   Gprasp1     Status: ES cell colonies screened / QC positives

GeneGprasp1
ChromosomeX
Deletion Start135,799,063
Deletion End135,803,130
Deletion Size4,068
Genome Build38
MGI IDMGI:1917418
NCBI Gene ID67298
BAC ID267G14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,799,844135,799,864GGCCCAAAGACAAGACCAAAG
TUP135,799,867135,799,891TGGTCTAAGCCCAAAGAAGAGCCCA
TUR135,799,930135,799,952TCAGACCCAGAAGTGGATTCAAC
TDF135,802,452135,802,472GCGAGGTGCTTCTCAGTTTAC
TDP135,802,474135,802,499CGGGATTTCATTCGAAACTCGGGCGT
TDR135,802,527135,802,545CACTCGGGAGGACGGATAG

PCR Primers

PrimerSequence
SUTGCTGACCAGTGCAATCATC
SDCTGCTCACTTTCACATAGACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev42684 bp-++
TUFTUR109 bp++-
TDFTDR94 bp++-
NeoFwdSD442 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11669B-H2VGB6C57BL/6NTac
11669B-H5VGB6C57BL/6NTac
11669C-B7VGB6C57BL/6NTac
11669C-D10VGB6C57BL/6NTac
11669C-E8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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