| Gene | Slc4a11 |
| Chromosome | 2 |
| Deletion Start | 130,696,083 |
| Deletion End | 130,691,504 |
| Deletion Size | 4,580 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,695,907 | 130,695,926 | CCGTGTGGCTTGCTGAGTAG |
| TUP | 130,695,881 | 130,695,905 | CAGCTGATGAGACTGGCCAAAGGCA |
| TUR | 130,695,844 | 130,695,864 | AATCTGCCCTCCCAAGACCTC |
| TDF | 130,691,628 | 130,691,647 | GCTTTGCCCTGGACCCTAAC |
| TDP | 130,691,603 | 130,691,627 | CACGCAGAACCTGACTGTGACCTGG |
| TDR | 130,691,542 | 130,691,563 | GCCTTACCCTTGCTCTCCATAG |
PCR Primers
| Primer | Sequence |
| SU | CCATACTAGGCAGACTAAGG |
| SD | TGGAGATGCCATCACAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11670B-E8 | VGB6 | C57BL/6NTac | |
| 11670C-C6 | VGB6 | C57BL/6NTac | |
| 11670C-F3 | VGB6 | C57BL/6NTac | |
| 11670C-G9 | VGB6 | C57BL/6NTac | |
| 11670B-A4 | VGB6 | C57BL/6NTac | |
